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Sci Rep. 2019 Jul 29;9(1):10923. doi: 10.1038/s41598-019-45588-z.

Enterotype-based Analysis of Gut Microbiota along the Conventional Adenoma-Carcinoma Colorectal Cancer Pathway.

Yang TW1,2,3, Lee WH3,4,5, Tu SJ4, Huang WC3,4,5, Chen HM4, Sun TH3, Tsai MC1,2,6, Wang CC1,2,6, Chen HY1,2, Huang CC2,7, Shiu BH6,7, Yang TL3, Huang HT3, Chou YP4, Chou CH3,4, Huang YR4, Sun YR4, Liang C4, Lin FM4, Ho SY3,4, Chen WL3, Yang SF5,6, Ueng KC2,5, Huang HD8,9,10,11,12, Huang CN2,13, Jong YJ14,15,16,17, Lin CC18,19.

Author information

1
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, 402, Taiwan.
2
School of Medicine, Chung Shan Medical University, Taichung, 402, Taiwan.
3
Institute and Department of Biological Science and Technology, College of Biological Science and Technology, National Chiao Tung University, Hsinchu, 300, Taiwan.
4
Institute of Bioinformatics and Systems Biology, College of Biological Science and Technology, National Chiao Tung University, Hsinchu, 300, Taiwan.
5
Department of Medical Research, Chung Shan Medical University Hospital, Taichung, 402, Taiwan.
6
Institute of Medicine, Chung Shan Medical University, Taichung, 402, Taiwan.
7
Division of Colon and Rectum, Department of Surgery, Chung Shan Medical University Hospital, Taichung, 402, Taiwan.
8
Institute and Department of Biological Science and Technology, College of Biological Science and Technology, National Chiao Tung University, Hsinchu, 300, Taiwan. huanghsienda@cuhk.edu.cn.
9
Institute of Bioinformatics and Systems Biology, College of Biological Science and Technology, National Chiao Tung University, Hsinchu, 300, Taiwan. huanghsienda@cuhk.edu.cn.
10
Warshel Institute For Computational Biology, The Chinese University of Hong Kong, Shenzhen, 518172, Longgang District, Shenzhen, China. huanghsienda@cuhk.edu.cn.
11
School of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, 518172, Longgang District, Shenzhen, China. huanghsienda@cuhk.edu.cn.
12
School of Sciences and Engineering, The Chinese University of Hong Kong, Shenzhen, 518172, Longgang District, Shenzhen, China. huanghsienda@cuhk.edu.cn.
13
Division of Endocrinology and Metabolism, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, 402, Taiwan.
14
Institute and Department of Biological Science and Technology, College of Biological Science and Technology, National Chiao Tung University, Hsinchu, 300, Taiwan. yjjong@kmu.edu.tw.
15
Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, 807, Taiwan. yjjong@kmu.edu.tw.
16
Departments of Pediatrics and Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, 807, Taiwan. yjjong@kmu.edu.tw.
17
Institute of Molecular Medicine and Bioengineering, College of Biological Science and Technology, National Chiao Tung University, Hsinchu, 300, Taiwan. yjjong@kmu.edu.tw.
18
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, 402, Taiwan. forest65@csmu.edu.tw.
19
School of Medicine, Chung Shan Medical University, Taichung, 402, Taiwan. forest65@csmu.edu.tw.

Abstract

The dysbiosis of human gut microbiota is strongly associated with the development of colorectal cancer (CRC). The dysbiotic features of the transition from advanced polyp to early-stage CRC are largely unknown. We performed a 16S rRNA gene sequencing and enterotype-based gut microbiota analysis study. In addition to Bacteroides- and Prevotella-dominated enterotypes, we identified an Escherichia-dominated enterotype. We found that the dysbiotic features of CRC were dissimilar in overall samples and especially Escherichia-dominated enterotype. Besides a higher abundance of Fusobacterium, Enterococcus, and Aeromonas in all CRC faecal microbiota, we found that the most notable characteristic of CRC faecal microbiota was a decreased abundance of potential beneficial butyrate-producing bacteria. Notably, Oscillospira was depleted in the transition from advanced adenoma to stage 0 CRC, whereas Haemophilus was depleted in the transition from stage 0 to early-stage CRC. We further identified 7 different CAGs by analysing bacterial clusters. The abundance of microbiota in cluster 3 significantly increased in the CRC group, whereas that of cluster 5 decreased. The abundance of both cluster 5 and cluster 7 decreased in the Escherichia-dominated enterotype of the CRC group. We present the first enterotype-based faecal microbiota analysis. The gut microbiota of colorectal neoplasms can be influenced by its enterotype.

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