Format

Send to

Choose Destination
Int J Mol Sci. 2019 Jul 26;20(15). pii: E3651. doi: 10.3390/ijms20153651.

MicroRNA Profiling During Neural Differentiation of Induced Pluripotent Stem Cells.

Author information

1
Radiobiology Lab, Department of Medical Physics, Greater Poland Cancer, 61-866 Poznań, Poland. katarzyna.kulcenty@wco.pl.
2
Department of Pathology, Poznan University of Medical Sciences and Greater Poland Cancer Center, Garbary 15th Street, 61-866 Poznan, Poland.
3
Department of Histology and Embryology, Poznan University of Medical Sciences, Swiecickiego 6 Street, 60-781 Poznan, Poland.
4
Department of Molecular Biomedicine, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14 Str., 61-704 Poznan, Poland.
5
Radiobiology Lab, Department of Medical Physics, Greater Poland Cancer, 61-866 Poznań, Poland.
6
Department of Electroradiology, Poznan University of Medical Sciences, Garbary 15th Street, 61-866 Poznan, Poland.

Abstract

MicroRNAs (miRNA) play an essential role in the regulation of gene expression and influence signaling networks responsible for several cellular processes like differentiation of pluripotent stem cells. Despite several studies on the neurogenesis process, no global analysis of microRNA expression during differentiation of induced pluripotent stem cells (iPSC) to neuronal stem cells (NSC) has been done. Therefore, we compared the profile of microRNA expression in iPSC lines and in NSC lines derived from them, using microarray-based analysis. Two different protocols for NSC formation were used: Direct and two-step via neural rosette formation. We confirmed the new associations of previously described miRNAs in regulation of NSC differentiation from iPSC. We discovered upregulation of miR-10 family, miR-30 family and miR-9 family and downregulation of miR-302 and miR-515 family expression. Moreover, we showed that miR-10 family play a crucial role in the negative regulation of genes expression belonging to signaling pathways involved in neural differentiation: WNT signaling pathway, focal adhesion, and signaling pathways regulating pluripotency of stem cells.

KEYWORDS:

human induced pluripotent stem cells; microRNA; neural differentiation; neural stem cells

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center