Format

Send to

Choose Destination
Nucleic Acids Res. 1988 Jul 11;16(13):5727-40.

Site-directed mutagenesis in the DNA linking site of bacteriophage phi 29 terminal protein: isolation and characterization of a Ser232----Thr mutant.

Author information

1
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.

Abstract

By site-directed mutagenesis we have changed the serine residue 232 of the phi 29 terminal protein, involved in the covalent linkage to dAMP for the initiation of replication, into a threonine residue. The mutant terminal protein has been purified to homogeneity and shown to be inactive in the formation of the initiation complex; nevertheless, the mutant protein retains its ability to interact with the phi 29 DNA polymerase and with the DNA. The results obtained indicate a high specificity in the linking site of the terminal protein.

PMID:
3135531
PMCID:
PMC336825
DOI:
10.1093/nar/16.13.5727
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center