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Stem Cell Res. 2019 Aug;39:101506. doi: 10.1016/j.scr.2019.101506. Epub 2019 Jul 18.

Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4+T cells of healthy individuals of Indian origin.

Author information

1
National Centre for Cell Science (NCCS), Pune 411007, India.
2
National Centre for Cell Science (NCCS), Pune 411007, India. Electronic address: anjali@nccs.res.in.

Abstract

Human induced pluripotent stem cell-lines (iPSCs) of Indian origin NCCSi005A and NCCSi006A were established by reprogramming of CD4+T cells, isolated from the peripheral blood mononuclear cells (PBMCs) of two healthy female donors. Reprogramming was achieved using integration free, Sendai viral vector system expressing cocktail of transcription factors KOS, hc-MYC and hKLF4. Both the established cell-lines showed alkaline phosphatase activity, expressed stemness markers, exhibited normal female karyotype and displayed potential for tri-lineage differentiation. These two CD4+T cells derived cell-lines represent valuable resource as control iPSC cell lines of Indian origin.

PMID:
31352200
DOI:
10.1016/j.scr.2019.101506
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