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FEBS J. 2019 Jul 26. doi: 10.1111/febs.15016. [Epub ahead of print]

Arabidopsis thaliana Hcc1 is a Sco-like metallochaperone for CuA assembly in Cytochrome c Oxidase.

Author information

1
Instituto de Biología Molecular y Celular de Rosario (IBR CONICET-UNR), Rosario, Argentina.
2
Plataforma de Biología Estructural y Metabolómica (PLABEM), Rosario, Argentina.
3
Area Biofísica, Departamento de Química Biológica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina.
4
Unité de Microbiologie Structurale, Institut Pasteur, Université Paris Diderot, Paris, France.

Abstract

The assembly of the CuA site in Cytochrome c Oxidase (COX) is a critical step for aerobic respiration in COX-dependent organisms. Several gene products have been associated with the assembly of this copper site, the most conserved of them belonging to the Sco family of proteins, which have been shown to perform different roles in different organisms. Plants express two orthologs of Sco proteins: Hcc1 and Hcc2. Hcc1 is known to be essential for plant development and for COX maturation, but its precise function has not been addressed until now. Here, we report the biochemical, structural and functional characterization of Arabidopsis thaliana Hcc1 protein (here renamed Sco1). We solved the crystal structure of the Cu+1 -bound soluble domain of this protein, revealing a tri coordinated environment involving a CxxxCxn H motif. We show that AtSco1 is able to work as a copper metallochaperone, inserting two Cu+1 ions into the CuA site in a model of CoxII. We also show that AtSco1 does not act as a thiol-disulfide oxido-reductase. Overall, this information sheds new light on the biochemistry of Sco proteins, highlighting the diversity of functions among them despite their high structural similarities. DATABASE: PDB entry 6N5U (Crystal structure of Arabidopsis thaliana ScoI with copper bound).

KEYWORDS:

CuA; Cytochrome-c-Oxidase; Sco; copper; metallochaperone

PMID:
31348612
DOI:
10.1111/febs.15016

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