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Methods Mol Biol. 2019;2030:253-261. doi: 10.1007/978-1-4939-9639-1_19.

High-Throughput LC-MS/MS Method for Chiral Amino Acid Analysis Without Derivatization.

Author information

1
Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan.
2
Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan. fukusaki@bio.eng.osaka-u.ac.jp.

Abstract

D-Amino acids have recently attracted much attention in various research fields including medical, clinical, and food industry due to their important biological functions that differ from L-amino acid. Most chiral amino acid separation techniques require complicated derivatization procedures in order to achieve the desirable chromatographic behavior and detectability. This chapter describes a highly sensitive analytical method for the enantioseparation of chiral amino acids without any derivatization process using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method allows the simultaneous analysis of 18 D-amino acids with high sensitivity and reproducibility. Additionally, this chapter also focuses on the application of the method to real samples for the quantification of targeted amino acids.

KEYWORDS:

Chiral; D-Amino acid; Enantioseparation; LC-MS/MS; Quantitative analysis; Underivatized

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