Format

Send to

Choose Destination
Exp Cell Res. 1988 Jul;177(1):122-31.

Reversible arrest of proliferation of rat 3Y1 fibroblasts in both the G1 and G2 phases by trichostatin A.

Author information

1
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.

Abstract

Proliferation of rat 3Y1 cells was found to be specifically blocked by trichostatin A (TSA) at two distinct stages in the cell cycle. The first block occurred in the early G1 phase at least 9 h before the G1/S boundary, whereas the second occurred during the G2 phase. When TSA-arrested cells at the G2 phase were released from the inhibition, the cells with 4C DNA entered a new S phase without passage through the M phase, resulting in the formation of proliferative tetraploid cells. The removal of TSA induced a rapid transient increase in the transcription of c-fos and the cells required 15 h to enter the S phase after release. These results suggest that the cells arrested with TSA are quiescent (G0).

PMID:
3134246
DOI:
10.1016/0014-4827(88)90030-4
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center