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Int J Mol Sci. 2019 Jul 14;20(14). pii: E3456. doi: 10.3390/ijms20143456.

Human Skeletal Muscle Cells Derived from the Orbicularis Oculi Have Regenerative Capacity for Duchenne Muscular Dystrophy.

Author information

1
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
2
Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan.
3
Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
4
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan. musclestemcell@gmail.com.
5
Department of Anatomy, Fujita Health University, Toyoake 470-1192, Japan. musclestemcell@gmail.com.
6
AMED-CREST, AMED 1-7-1 Otemachi, Chiyoda, Tokyo 100-0004, Japan. musclestemcell@gmail.com.

Abstract

Skeletal muscle stem cells (MuSCs) have been proposed as suitable candidates for cell therapy in muscular disorders since they exhibit good capacity for myogenic regeneration. However, for better therapeutic outcomes, it is necessary to isolate human MuSCs from a suitable tissue source with high myogenic differentiation. In this context, we isolated CD56+CD82+ cells from the extra eyelid tissue of young and aged patients, and tested in vitro myogenic differentiation potential. In the current study, myogenic cells derived from extra eyelid tissue were characterized and compared with immortalized human myogenic cells. We found that myogenic cells derived from extra eyelid tissue proliferated and differentiated myofibers in vitro, and restored DYSTROPHIN or PAX7 expression after transplantation with these cells in mice with Duchenne muscular dystrophy. Thus, human myogenic cells derived from extra eyelid tissue including the orbicularis oculi might be good candidates for stem cell-based therapies for treating muscular diseases.

KEYWORDS:

Duchenne muscular dystrophy; cell transplantation; muscle stem cell; orbicularis oculi

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