Format

Send to

Choose Destination
Allergy Asthma Immunol Res. 2019 Sep;11(5):691-708. doi: 10.4168/aair.2019.11.5.691.

In-Depth, Proteomic Analysis of Nasal Secretions from Patients With Chronic Rhinosinusitis and Nasal Polyps.

Kim YS#1,2, Han D#3, Kim J4, Kim DW5,6, Kim YM7,6, Mo JH8,6, Choi HG9, Park JW1,2,10,11, Shin HW1,2,10,11,12,13.

Author information

1
Obstructive Upper airway Research (OUaR) Laboratory, Department of Pharmacology, Seoul National University College of Medicine, Seoul, Korea.
2
Department of Biomedical Sciences, Seoul National University Graduate School, Seoul, Korea.
3
Proteomics core facility, Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea.
4
Department of Otorhinolaryngology, Armed Forces Capital Hospital, Seongnam, Korea.
5
Department of Otorhinolaryngology-Head and Neck Surgery, Boramae Medical Center, Seoul, Korea.
6
Clinical Mucosal Immunology Study Group, Seoul, Korea.
7
Department of Otorhinolaryngology-Head and Neck Surgery, Chungnam National University Hospital, Daejeon, Korea.
8
Department of Otorhinolaryngology-Head and Neck Surgery, Dankook University Hospital, Cheonan, Korea.
9
Department of Otorhinolaryngology-Head and Neck Surgery, Hallym University Hospital, Pyongchon, Korea.
10
Ischemic/hypoxic disease institute, Seoul National University College of Medicine, Seoul, Korea.
11
Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.
12
Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul, Korea.
13
Clinical Mucosal Immunology Study Group, Seoul, Korea. charlie@snu.ac.kr.
#
Contributed equally

Abstract

PURPOSE:

Chronic rhinosinusitis (CRS) is a complex immunological condition, and novel experimental modalities are required to explore various clinical and pathophysiological endotypes; mere evaluation of nasal polyp (NP) status is inadequate. Therefore, we collected patient nasal secretions on filter paper and characterized the proteomes.

METHODS:

We performed liquid chromatography-mass spectrometry (MS)/MS in the data-dependent acquisition (DDA) and data-independent acquisition (DIA) modes. Nasal secretions were collected from 10 controls, 10 CRS without NPs (CRSsNP) and 10 CRS with NPs (CRSwNP). We performed Orbitrap MS-based proteomic analysis in the DDA (5 controls, 5 CRSsNP and 5 CRSwNP) and the DIA (5 controls, 5 CRSsNP and 5 CRSwNP) modes, followed by a statistical analysis and a hierarchical clustering to identify differentially expressed proteins in the 3 groups.

RESULTS:

We identified 2,020 proteins in nasal secretions. Canonical pathway analysis and gene ontology (GO) evaluation revealed that interleukin (IL)-7, IL-9, IL-17A and IL-22 signaling and neutrophil-mediated immune responses like neutrophil degranulation and activation were significantly increased in CRSwNP compared to control. The GO terms related to the iron ion metabolism that may be associated with CRS and NP development.

CONCLUSIONS:

Collection of nasal secretions on the filter paper is a practical and non-invasive method for in-depth study of nasal proteomics. Our proteomic signatures also support that Asian NPs could be characterized as non-eosinophilic inflammation features. Therefore, the proteomic profiling of nasal secretions from CRS patients may enhance our understanding of CRS endotypes.

KEYWORDS:

Sinusitis; nasal polyps; proteomics

PMID:
31332980
PMCID:
PMC6658407
[Available on 2019-09-01]
DOI:
10.4168/aair.2019.11.5.691
Free full text

Supplemental Content

Full text links

Icon for The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease
Loading ...
Support Center