Format

Send to

Choose Destination
Neuropharmacology. 2019 Jul 17;158:107716. doi: 10.1016/j.neuropharm.2019.107716. [Epub ahead of print]

Cannabidiol improves vocal learning-dependent recovery from, and reduces magnitude of deficits following, damage to a cortical-like brain region in a songbird pre-clinical animal model.

Author information

1
Department of Pharmacology and Toxicology, ECU Brody School of Medicine, Greenville, NC, 27834, USA; Department of Pharmacology and Toxicology, Pharmacy College, Taibah University, Medina, Saudi Arabia.
2
Department of Pharmacology and Toxicology, ECU Brody School of Medicine, Greenville, NC, 27834, USA.
3
GW Research Ltd, Sovereign House, Vision Park, Histon, Cambridge, CB24 9BZ, UK.
4
GW Research Ltd, Sovereign House, Vision Park, Histon, Cambridge, CB24 9BZ, UK; Endocannabinoid Research Group, Institute of Biomolecular Chemistry, Consiglio Nazionale delle Ricerche, Via Campi Flegrei, Pozzuoli (NA), 34- 80078, Italy.
5
Department of Pharmacology and Toxicology, ECU Brody School of Medicine, Greenville, NC, 27834, USA. Electronic address: soderstromk@ecu.edu.

Abstract

Cannabidiol (CBD), a non-euphorigenic compound derived from Cannabis, shows promise for improving recovery following cerebral ischemia and has recently been shown effective for the treatment of childhood seizures caused by Dravet and Lennox-Gastaut syndromes. Given evidence for activity to mitigate effects of CNS insult and dysfunction, we considered the possibility that CBD may also protect and improve functional recovery of a complex learned behavior. To test this hypothesis, we have applied a songbird, the adult male zebra finch, as a novel pre-clinical animal model. Their learned vocalizations were temporarily disrupted with bilateral microlesions of HVC (used as a proper name) a pre-vocal motor cortical-like brain region that drives song. These microlesions destroy about 10% of HVC, and temporarily impair song production, syntax and phonology for about seven days. Recovery requires sensorimotor learning as it depends upon auditory feedback. Four CBD doses (0, 1, 10 and 100 mg/kg) within three surgery conditions (microlesion, no-microlesion, sham-microlesion) were evaluated (n = 5-6). Birds were recorded over 20 days: three baseline; six pre-microlesion drug treatment days and; 11 post-microlesion treatment and recovery days. Results indicate 10 and 100 mg/kg CBD effectively reduced the time required to recover vocal phonology and syntax. In the case of phonology, the magnitude of microlesion-related disruptions were also reduced. These results suggest CBD holds promise to improve functional recovery of complex learned behaviors following brain injury, and represent establishment of an important new animal model to screen drugs for efficacy to improve vocal recovery.

KEYWORDS:

Animal model; CNS lesion; Cannabinoid; Vocal learning

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center