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J AOAC Int. 2019 Jul 18. doi: 10.5740/jaoacint.18-0238. [Epub ahead of print]

Application of Sample6 DETECT™ HT/L Kit for the Detection of Listeria in Mixed Leafy Greens.

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Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155.
IEH Laboratories and Consulting Group, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155.
Microbiologique, Inc. 8215 Lake City Way NE, Seattle WA 98115.


Background: Early and accurate detection of Listeria in foods is vital. Current methods require 24 h enrichment for detection. Objective: This study aimed to demonstrate enhanced early detection of Listeria in mixed leafy greens using Sample6 DETECT HT/L, a phage-based detection system. Methods: A method comparison between the reference method (U.S. Food and Drug Administration Bacteriological Analytical Manual Chapter 10) for the detection of Listeria spp. and the Sample6 DETECT HT/L using a new proprietary R2 Medium was performed in mixed leafy greens. Results: Using the R2 Medium enrichment with Sample6 DETECT HT/L, detection of L. innocua was possible at 12 h (with a centrifugation step), and L. monocytogenes was detected by 18 h, with equivalent performance as the 24 h enrichments using the reference method detection. The Sample6 DETECT HT/L gave an equivalent performance for L. innocua at 15 h as the reference method at 24 h. Detection was accomplished by the addition of reagents in the kit, following the package insert, and reading results in a detection chamber using a 96-well plate reader that detects a fluorescent signal. Conclusions: Results indicate the new R2 Medium and Sample6 DETECT HT/L allowed for earlier detection of Listeria spp. in mixed leafy greens. Highlights: Sample6 DETECT HT/L with the new R2 Medium allowed the early detection of Listeria spp. and may be applied in other food matrices and environmental samples.


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