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J Immunol Methods. 1988 May 25;110(1):29-36.

Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells.

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1
Department of Oral Microbiology, University of Göteborg, Sweden.

Abstract

A reverse modification of ELISPOT assay using nitrocellulose membranes and epitope-specific monoclonal antibodies is described for the detection of single lymphokine-secreting cells. As a model, the production of gamma-interferon by mitogen stimulated human peripheral blood lymphocytes has been examined. The assay can also be modified to permit microscopic examination of spot-forming cells.

PMID:
3131436
DOI:
10.1016/0022-1759(88)90079-8
[Indexed for MEDLINE]

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