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Neurophotonics. 2019 Oct;6(4):041103. doi: 10.1117/1.NPh.6.4.041103. Epub 2019 Jul 9.

Longitudinal detection of retinal alterations by visible and near-infrared optical coherence tomography in a dexamethasone-induced ocular hypertension mouse model.

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Boston University School of Medicine, Boston Medical Center, Department of Medicine, Boston, Massachusetts, United States.
Boston University, Department of Biology, Boston, Massachusetts, United States.
Boston University Sargent School of Rehabilitation, Department of Health Science, Boston, Massachusetts, United States.
Dana-Farber Cancer Institute, Boston, Massachusetts, United States.
Boston Medical Center, Department of Ophthalmology, Boston, Massachusetts, United States.
Boston University, Department of Biomedical Engineering, Boston, Massachusetts, United States.


The retina, as part of the central nervous system, has distinct anatomical and structural properties for its visual function. Light scattering spectroscopy, while widely used for tissue structural characterization and disease diagnosis, has been relatively unexplored in the living retina. Recently, we have developed a fiber-based visible and near-infrared optical coherence tomography system (vnOCT) for in vivo retinal imaging, to uniquely measure a spectroscopic marker (VN ratio) sensitive to nanoscale pathological changes. In the present study, we applied vnOCT in an animal model of glaucoma (dexamethasone-induced ocular hypertension mouse) and tested the capabilities of four optical markers, VN ratio, peripapillary retinal nerve fiber layer (RNFL) thickness, total retinal blood flow, and hemoglobin oxygen saturation ( sO 2 ), for the detection of retinal ganglion cell (RGC) damage in association with ocular hypertension. We found that RNFL-RGC VN ratio and arteriovenous (A-V) sO 2 are capable of detecting early retinal alteration in ocular hypertensive eyes, preceding measurable change of RNFL thickness. This study suggests a potential clinical application of vnOCT in early detection of glaucoma.


VN ratio; hemoglobin oxygen saturation; ocular hypertension; visible and near-infrared optical coherence tomography system

[Available on 2020-07-09]
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