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R Soc Open Sci. 2019 Jun 26;6(6):190266. doi: 10.1098/rsos.190266. eCollection 2019 Jun.

Dendropanax morbiferus leaf extract facilitates oligodendrocyte development.

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Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine, Seoul 120-749, Republic of Korea.
Department of Integrative Biosciences, University of Brain Education, Cheonan 31228, Republic of Korea.
Department of Biochemistry and Integrative Medical Biology, School of Medicine, Keio University, Tokyo 160-8582, Japan.
Department of Health Science and Daily Sports, Global Cyber University, Cheonan 31228, Republic of Korea.
Cheju Halla University, Jeju 63092, Republic of Korea.
Department of Medical Science, International St Mary's Hospital, Catholic Kwandong University, Gangneung, Republic of Korea.
Korea Institute of Brain Science, Seoul, Republic of Korea.


Treatment of multiple sclerosis is effective when anti-inflammatory, neuroprotective and regenerative strategies are combined. Dendropanax morbiferus (DM) has anti-inflammatory, anti-oxidative properties, which may be beneficial for multiple sclerosis. However, there have been no reports on the effects of DM on myelination, which is critical for regenerative processes. To know whether DM benefits myelination, we checked differentiation and myelination of oligodendrocytes (OLs) in various primary culture systems treated with DM leaf EtOH extracts or control. DM extracts increased the OL membrane size in the mixed glial and pure OL precursor cell (OPC) cultures and changed OL-lineage gene expression patterns in the OPC cultures. Western blot analysis of DM-treated OPC cultures showed upregulation of MBP and phosphorylation of ERK1/2. In myelinating cocultures, DM extracts enhanced OL differentiation, followed by increased axonal contacts and myelin gene upregulations such as Myrf, CNP and PLP. Phytochemical analysis by LC-MS/MS identified multiple components from DM extracts, containing bioactive molecules such as quercetin, cannabidiol, etc. Our results suggest DM extracts enhance OL differentiation, followed by an increase in membrane size and axonal contacts, thereby indicating enhanced myelination. In addition, we found that DM extracts contain multiple bioactive components, warranting further studies in relation to finding effective components for enhancing myelination.


Dendropanax morbiferus; differentiation; multiple sclerosis; myelin; oligodendrocyte

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