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New Phytol. 2019 Jul 16. doi: 10.1111/nph.16065. [Epub ahead of print]

Lateral root initiation requires the sequential induction of transcription factors LBD16 and PUCHI in Arabidopsis thaliana.

Author information

1
Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai, Kobe, 657-8501, Japan.
2
Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, 630-0192, Japan.
3
Department of Biological Sciences, Graduate School of Science, Osaka University, 13 Toyonaka, Osaka, 560-0043, Japan.
4
Faculty of Science and Engineering, Konan University, Kobe, 658-5801, Japan.
5
Graduate School of Science and Technology, Kobe University, 1-1 Rokkodai, Kobe, 657-8501, Japan.
6
Department of Bioregulation and Biointeraction, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, 183-8509, Japan.
7
Center for Sustainable Resource Science, Riken, Yokohama, Kanagawa, 230-0045, Japan.
8
Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, 183-8509, Japan.

Abstract

Lateral root (LR) formation in Arabidopsis thaliana is initiated by asymmetric division of founder cells, followed by coordinated cell proliferation and differentiation for patterning new primordia. The sequential developmental processes of LR formation are triggered by a localized auxin response. LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16), an auxin-inducible transcription factor, is one of the key regulators linking auxin response in LR founder cells to LR initiation. We identified key genes for LR formation that are activated by LBD16 in an auxin-dependent manner. LBD16 targets identified include the transcription factor gene PUCHI, which is required for LR primordium patterning. We demonstrate that LBD16 activity is required for the auxin-inducible expression of PUCHI. We show that PUCHI expression is initiated after the first round of asymmetric cell division of LR founder cells and that premature induction of PUCHI during the preinitiation phase disrupts LR primordium formation. Our results indicate that LR initiation requires the sequential induction of transcription factors LBD16 and PUCHI.

KEYWORDS:

Arabidopsis thaliana ; LBD16 ; PUCHI ; auxin; lateral root

PMID:
31310684
DOI:
10.1111/nph.16065

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