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Nat Methods. 2019 Aug;16(8):699-702. doi: 10.1038/s41592-019-0468-x. Epub 2019 Jul 15.

A genetically encodable cell-type-specific protein synthesis inhibitor.

Author information

1
Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
2
Max Planck Institute for Brain Research, Frankfurt am Main, Germany. erin.schuman@brain.mpg.de.

Abstract

Chemical inhibitors have revealed requirements for protein synthesis that drive cellular plasticity. We developed a genetically encodable protein synthesis inhibitor (gePSI) to achieve cell-type-specific temporal control of protein synthesis. Controlled expression of the gePSI in neurons or glia resulted in rapid, potent and reversible cell-autonomous inhibition of protein synthesis. Moreover, gePSI expression in a single neuron blocked the structural plasticity induced by single-synapse stimulation.

PMID:
31308551
DOI:
10.1038/s41592-019-0468-x

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