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J Am Soc Nephrol. 2019 Oct;30(10):1870-1885. doi: 10.1681/ASN.2018101067. Epub 2019 Jul 11.

Anti-CD45RB Antibody Therapy Attenuates Renal Ischemia-Reperfusion Injury by Inducing Regulatory B Cells.

Author information

1
Transplantation Research Institute and.
2
Department of Medicine, Graduate School, Seoul National University College of Medicine, Seoul, Republic of Korea.
3
Transplantation Center and.
4
Asan Institute for Life Sciences, Asan Medical Center, Seoul, Republic of Korea; and.
5
Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul, Republic of Korea.
6
Department of Medicine, Graduate School, Seoul National University College of Medicine, Seoul, Republic of Korea; jcyjs@snu.ac.kr kssuh@snu.ac.kr.
7
Department of Surgery, Seoul National University Hospital, Seoul, Republic of Korea.
8
Transplantation Research Institute and jcyjs@snu.ac.kr kssuh@snu.ac.kr.

Abstract

BACKGROUND:

Regulatory B cells are a newly discovered B cell subset that suppresses immune responses. Recent studies found that both anti-CD45RB and anti-Tim-1 treatments regulate immune responses by inducing regulatory B cells; however, the role of these cells in renal ischemia-reperfusion injury (IRI) is unknown.

METHODS:

Using mouse models, including T cell-deficient (RAG1 knockout and TCRα knockout) mice and B cell-deficient (μMT) mice, we investigated the effects of regulatory B cells and anti-CD45RB on IRI and the mechanisms underlying these effects.

RESULTS:

Adoptive transfer of regulatory B cells before or after IRI attenuated renal IRI. Anti-CD45RB treatment with or without anti-Tim-1 before IRI increased renal infiltration of CD19+Tim-1+ regulatory B and regulatory T cells. Anti-CD45RB decreased serum creatinine levels, pathologic injury score, tubular apoptosis, and proinflammatory cytokines levels, whereas IL-10 levels increased. Following IRI, anti-CD45RB with or without anti-Tim-1 also induced regulatory B cells, improving renal function and tubular regeneration. In RAG1 knockout mice with B cell transfer, TCRα knockout mice, and wild-type mice with T cell depletion, anti-CD45RB increased regulatory B cells and attenuated IRI. However, anti-CD45RB did not attenuate IRI in RAG1 knockout mice with T cell transfer or μMT mice and induced only mild improvement in wild-type mice with B cell depletion. Furthermore, B cell-deficient mice receiving B cells from IL-10 knockout mice (but not from wild-type mice) did not show renal protection against IRI when treated with anti-CD45RB.

CONCLUSIONS:

Anti-CD45RB treatment attenuated acute renal injury and facilitated renal recovery after IRI through induction of IL-10+ regulatory B cells, pointing to anti-CD45RB as a potential therapeutic strategy in renal IRI.

KEYWORDS:

anti-CD45RB; anti-Tim-1; ischemia-reperfusion injury; regulatory b cell

PMID:
31296607
DOI:
10.1681/ASN.2018101067

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