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Angew Chem Int Ed Engl. 2019 Jul 11. doi: 10.1002/anie.201907410. [Epub ahead of print]

Direct Visualization of Live Zebrafish Glycan via Single-step Metabolic Labeling with Fluorophore-tagged Nucleotide Sugars.

Author information

1
UNITED STATES.
2
The Scripps Researcdh Institute, Department of Chemical Physiology, 10550 N. Torrey Pines RD., 92037, La Jolla, UNITED STATES.

Abstract

Dynamic turnover of cell-surface glycans is involved in a myriad of biological events, making this process an attractive target for in vivo molecular imaging. Metabolic glycan labeling coupled with 'bioorthogonal chemistry' has paved the way for visualizing glycans in living organisms. However, a two-step labeling sequence is required, which is prone to tissue penetration difficulties for the imaging probes. Here, by exploring the substrate promiscuity of endogenous glycosyltransferases, we developed a single-step fluorescent glycan labeling strategy by directly using fluorophore-tagged analogs of the nucleotide sugars. Injecting the fluorophore-tagged sialic acid and fucose into the yolk of zebrafish embryos at the one-cell stage enables systematic imaging of sialylation and fucosylation in live zebrafish embryos at distinct developmental stages. From these studies, we obtained insights into the role of sialylated and fucosylated glycans in zebrafish hematopoiesis.

KEYWORDS:

Glycan labeling Fluorophore-tagged nucleotide sugar sialylation fucosylation hematopoiesis

PMID:
31295389
DOI:
10.1002/anie.201907410

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