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J Immunol Methods. 1988 May 9;109(2):215-9.

Non-specific propagation of human antigen-dependent T lymphocyte clones.

Author information

1
Department of Medical Microbiology and Immunology, University of Ulm, F.R.G.

Abstract

Most T lymphocyte clones require periodical restimulation with specific antigen in addition to growth factors to maintain long-term growth in vitro. Since in many cases the specific antigen for a given T cell clone is not known or not available, alternative methods of stimulation are required. In this report a method for a non-antigen-specific stimulation of antigen-dependent proliferative or cytotoxic human T cell clones is described. Introduction of aldehydes into galactose residues on stimulator cells leads to the stimulation of T cell clones by modified stimulator cells in the absence of a mitogen. This system is as efficient as the use of mitogenic antibodies or lectins but avoids the disadvantages of these polyclonal T cell activators.

PMID:
3129518
DOI:
10.1016/0022-1759(88)90245-1
[Indexed for MEDLINE]

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