Format

Send to

Choose Destination
Cell Biol Int. 2019 Jul 10. doi: 10.1002/cbin.11200. [Epub ahead of print]

Trolox-induced cardiac differentiation is mediated by the inhibition of Wnt/β-catenin signaling in human embryonic stem cells.

Author information

1
Department of Molecular Pharmacology, College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Daehak-ro, Daegu, 41566, Korea.
2
Department of Veterinary Physiology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Seoul, 08826, Korea.
3
Department of Cardiovascular and Neurologic Disease, College of Oriental Medicine, Daegu Haany University, 136 Sincheondong-ro, Daegu, 42158, Korea.
4
Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, 111 Geonjae-ro, Naju, Jeollanam-do 58245, Korea.
5
Futuristic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology, 30 Yeongudanji-ro, Cheongju, Chungcheongbuk-do 28116, Korea.
6
Department of Veterinary Pharmacology, College of Veterinary Medicine (BK21 Project Team), Chonnam National University, 77 Yongbong-ro, Gwangju, 61186, Korea.
7
Department of Biology Education, College of Education, Pusan National University, 2 Busandaehak-ro, Busan, 46241, Korea.
8
Department of Pharmacology, School of Medicine, Gyeongsang National University, 15 Jinjudae-ro 816, Jinju, 52727, Korea.
9
Department of Pharmacology, School of Medicine, Yale University, 333 Cedar street, New Haven, Connecticut, 06520, USA.

Abstract

Cardiac differentiation of human pluripotent stem cells may be induced under chemically defined conditions, wherein the regulation of Wnt/β-catenin pathway is often desirable. Here, we examined the effect of trolox, a vitamin E analog, on the cardiac differentiation of human embryonic stem cells (hESCs). 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) significantly enhanced cardiac differentiation in a time- and dose-dependent manner after the mesodermal differentiation of hESCs. Trolox promoted hESC cardiac differentiation through its inhibitory activity against the Wnt/β-catenin pathway. This study demonstrates an efficient cardiac differentiation method and reveals a novel Wnt/β-catenin regulator.

KEYWORDS:

cardiac differentiation; human embryonic stem cells; trolox

PMID:
31293030
DOI:
10.1002/cbin.11200

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center