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Cell. 1988 Apr 22;53(2):211-7.

Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor.

Author information

1
Whitehead Institute for Biomedical Research Cambridge, Massachusetts 02142.

Abstract

In cells that do not express kappa immunoglobulin light chain genes, the kappa enhancer-binding protein NF-kappa B is not evident in either cytoplasmic or nuclear fractions. By denaturation, size fractionation, and renaturation, however, NF-kappa B activity can be revealed in cytosolic fractions, showing that the DNA-binding protein is present but inhibited in its binding activity. By using a variety of protocols involving the dissociating agents sodium desoxycholate and formamide, as much cytosolic NF-kappa B can be found in the fraction from unstimulated 70Z/3 pre-B cells as is found in the nuclear extract from phorbol ester-activated cells. We conclude that both 70Z/3 and HeLa cells contain apparently cytosolic NF-kappa B in a form with no evident DNA-binding activity, and phorbol esters both release the inhibition of binding and cause a translocation to the nucleus.

PMID:
3129195
[Indexed for MEDLINE]

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