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Oncol Lett. 2019 Jul;18(1):189-196. doi: 10.3892/ol.2019.10288. Epub 2019 Apr 30.

Ampelopsin-sodium induces apoptosis in human lung adenocarcinoma cell lines by promoting tubulin polymerization in vitro.

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Department of Pharmacology, School of Basic Medical Science, Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
Department of Pharmacology, College of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou, Gansu 730000, P.R. China.
Department of Drug Policy and Essential Medicine, Xi'an Municipal Health Commission, Xi'an, Shaanxi 710000, P.R. China.


Previous studies have demonstrated that ampelopsin (AMP), a type of flavonoid isolated from the stems and leaves of Ampelopsis grossedentata, exhibits anti-cancer activity in various types of cancer. Conversion of AMP into its sodium salt (AMP-Na) conferred enhanced solubility and stability to it. The present study aimed to evaluate the anti-cancer activity of AMP-Na in human lung adenocarcinoma cell lines and to investigate its mechanisms of action. Cell proliferation and viability were assessed by MTT and colony formation assays, and cell migration was determined using a scratch wound healing assay. The cell cycle distribution, apoptosis rate and tubulin immunofluorescence intensity were analyzed using flow cytometry, the cell ultra-microstructure was examined using transmission electron microscopy and the accumulation of tubulin was determined using laser confocal microscopy. The results demonstrated that AMP-Na significantly inhibited the proliferation, clonogenicity and migration of human lung adenocarcinoma cells. Furthermore, AMP-Na induced SPC-A-1 cell apoptosis, and promoted tubulin polymerization. The results suggested that the underlying mechanisms of AMP-Na may involve targeting of microtubules and tubulin polymerization to subsequently disrupt mitosis and induce cell cycle arrest at the S-phase.


ampelopsin sodium; anti-tumor; apoptosis; human lung adenocarcinoma cell lines; tubulin polymerization

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