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Molecules. 2019 Jul 6;24(13). pii: E2482. doi: 10.3390/molecules24132482.

Nardochinoid B Inhibited the Activation of RAW264.7 Macrophages Stimulated by Lipopolysaccharide through Activating the Nrf2/HO-1 Pathway.

Yao YD1,2, Shen XY3, Machado J4, Luo JF1,2, Dai Y5, Lio CK1,2, Yu Y5, Xie Y1,2, Luo P1,2, Liu JX6, Yao XS3,5, Liu ZQ7, Zhou H8,9,10.

Author information

1
Faculty of Chinese Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China.
2
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China.
3
College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
4
ICBAS-Laboratory of Applied Physiology, Abel Salazar Institute of Biomedical Sciences, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
5
Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China.
6
College of Pharmacy, Hunan University of Chinese Medicine, Changsha 418000, China.
7
Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. liuzq@gzucm.edu.cn.
8
Faculty of Chinese Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China. hzhou@must.edu.mo.
9
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao 999078, China. hzhou@must.edu.mo.
10
Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. hzhou@must.edu.mo.

Abstract

Nardochinoid B (NAB) is a new compound isolated from Nardostachys chinensis. Although our previous study reported that the NAB suppressed the production of nitric oxide (NO) in lipopolysaccharide (LPS)-activated RAW264.7 cells, the specific mechanisms of anti-inflammatory action of NAB remains unknown. Thus, we examined the effects of NAB against LPS-induced inflammation. In this study, we found that NAB suppressed the LPS-induced inflammatory responses by restraining the expression of inducible nitric oxide synthase (iNOS) proteins and mRNA instead of cyclooxygenase-2 (COX-2) protein and mRNA in RAW264.7 cells, implying that NAB may have lower side effects compared with nonsteroidal anti-inflammatory drugs (NSAIDs). Besides, NAB upregulated the protein and mRNA expressions of heme oxygenase (HO)-1 when it exerted its anti-inflammatory effects. Also, NAB restrained the production of NO by increasing HO-1 expression in LPS-stimulated RAW264.7 cells. Thus, it is considered that the anti-inflammatory effect of NAB is associated with an induction of antioxidant protein HO-1, and thus NAB may be a potential HO-1 inducer for treating inflammatory diseases. Moreover, our study found that the inhibitory effect of NAB on NO is similar to that of the positive drug dexamethasone, suggesting that NAB has great potential for developing new drugs in treating inflammatory diseases.

KEYWORDS:

Nardostachys chinensis; heme oxygenase-1; inducible nitric oxide synthase; nardochinoid B; nitric oxide

PMID:
31284554
DOI:
10.3390/molecules24132482
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