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Curr Opin Struct Biol. 2019 Oct;58:259-268. doi: 10.1016/j.sbi.2019.05.022. Epub 2019 Jul 3.

Membrane mimetic systems in CryoEM: keeping membrane proteins in their native environment.

Author information

1
Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.
2
Department of Physiology, University of California, San Francisco, CA 94143, USA. Electronic address: David.Julis@ucsf.edu.
3
Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA; Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA. Electronic address: Yifan.Cheng@ucsf.edu.

Abstract

Advances in electron microscopes, detectors and data processing algorithms have greatly facilitated the structural determination of many challenging integral membrane proteins that have been evasive to crystallization. These breakthroughs facilitate the application and development of various membrane protein solubilization approaches for structural studies, including reconstitution into lipid nanoparticles. In this review, we discuss various approaches for preparing transmembrane proteins for structural determination with single-particle electron cryo microscopy (cryoEM).

PMID:
31279500
PMCID:
PMC6778513
[Available on 2020-10-01]
DOI:
10.1016/j.sbi.2019.05.022

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