Reshaping human antibodies for therapy

Nature. 1988 Mar 24;332(6162):323-7. doi: 10.1038/332323a0.

Abstract

A human IgGI antibody has been reshaped for serotherapy in humans by introducing the six hypervariable regions from the heavy- and light-chain variable domains of a rat antibody directed against human lymphocytes. The reshaped human antibody is as effective as the rat antibody in complement and is more effective in cell-mediated lysis of human lymphocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / genetics*
  • Antibodies, Monoclonal / therapeutic use
  • Base Sequence
  • Binding Sites, Antibody
  • Genes, Synthetic
  • Humans
  • Immunoglobulin G / genetics*
  • Immunoglobulin G / therapeutic use
  • Immunoglobulin Heavy Chains / genetics
  • Immunoglobulin Light Chains / genetics
  • Immunoglobulin Variable Region / genetics
  • Lymphocytes / immunology
  • Molecular Sequence Data
  • Rats
  • Recombinant Fusion Proteins / genetics*
  • Recombinant Fusion Proteins / therapeutic use
  • Recombinant Proteins / genetics*
  • Species Specificity

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin G
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Immunoglobulin Variable Region
  • Recombinant Fusion Proteins
  • Recombinant Proteins