Format

Send to

Choose Destination
Intern Med J. 2019 Jul 5. doi: 10.1111/imj.14419. [Epub ahead of print]

Pre- and post-bone marrow harvest anaemia is associated with lower CD34+ stem cell collection, high harvest volume and female gender.

Author information

1
Blood and Marrow Transplantation Program, Westmead Hospital, Sydney.
2
Sydney Cellular Therapies Laboratory, Westmead Hospital, Sydney.
3
University of Sydney, Australia.
4
Westmead Institute for Medical Research, University of Sydney, Australia.

Abstract

BACKGROUND:

Donor safety is paramount when performing bone marrow stem cell harvest. The incidence of full blood count (FBC) abnormalities among donors and variables associated with anaemia after marrow harvest are not well established.

AIMS:

To describe to frequency of FBC abnormalities prior to bone marrow stem cell harvest and to identify variable associated with post harvest anaemia.

METHODS:

Outcomes of 80 consecutive adult marrow harvests performed at our center were analyzed retrospectively.

RESULTS:

FBC abnormalities were present in 28% of donors prior to marrow harvest with normocytic anaemia the most common abnormality in 13%. Reduced donor Hb was independently correlated with lower CD34+ cell count per kg of recipient body weight. Anaemia (Hb<100g/L) was seen in 20% of donors after harvest with median decrease in Hb of 19g/L. Variables independently associated with anaemia after harvest included donor to recipient weight ratio (p=0.011), high collection volume (p=0.044) and female gender (p=0.023). Total nucleated cell and CD34 concentration in the final collected product were associated with the inverse of harvested marrow volume (p<0.001).

CONCLUSIONS:

Pre-harvest anaemia should be corrected where possible particularly in female donors. Marrow collection volume should be minimized to reduce post-harvest anaemia, optimize CD34+ cell number and improve nucleated and stem cell concentrations in the harvest product. This article is protected by copyright. All rights reserved.

PMID:
31276271
DOI:
10.1111/imj.14419

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center