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J Agric Food Chem. 2019 Jul 17;67(28):7855-7868. doi: 10.1021/acs.jafc.9b03082. Epub 2019 Jul 5.

Bee Pollen Extracts Modulate Serum Metabolism in Lipopolysaccharide-Induced Acute Lung Injury Mice with Anti-Inflammatory Effects.

Author information

1
Institute of Apicultural Research , Chinese Academy of Agricultural Sciences , Beijing 100093 , China.
2
Beijing Laboratory Animal Research Center , Beijing 100012 , China.
3
Department of Agricultural Food and Nutritional Science, Faculty of Agricultural, Life & Environmental Sciences , University of Alberta , Edmonton , Alberta T6G 2P5 , Canada.

Abstract

Bee pollen (BP) collected from different floras possesses various potential bioactivities, but the mechanism-related research on anti-inflammatory effects is limited. Here, three types of BP originating from Camellia sinensis L. (BP-Cs), Nelumbo nucifera Gaertn. (BP-Nn), and Brassica campestris L. (BP-Bc) were assessed using molecular and metabolomics methods to determine their anti-inflammatory effects. The differences in polyphenolic abundance of three types of BP extracts were determined by HPLC-DAD/Q-TOF-MS. In vitro anti-inflammatory effects of three BP extracts were evaluated in a lipopolysaccharide (LPS)-induced RAW 264.7 cells model. BP-Cs extract with the most abundant polyphenols was found to be the most effective in reducing inflammation by downregulating inflammatory-related genes expression and blocking the activation of MAPK and NF-κB signaling pathways. Polyphenol-rich BP-Cs was further evaluated for their in vivo anti-inflammatory effect in a LPS-induced acute lung injury mouse model. An UPLC-Q-TOF/MS-based metabolomics approach was applied to analyze metabolite changes in mouse serum. Weshowed that the pretreated BP-Cs extract alleviated inflammation and regulated glycerophospholipid metabolism significantly. Our findings provide a foundation for developing and justifying BP as a potential anti-inflammatory ingredient in functional foods or nutraceutical formulations.

KEYWORDS:

anti-inflammatory effect; bee pollen; metabolism regulation; metabolomics; polyphenols

PMID:
31274310
DOI:
10.1021/acs.jafc.9b03082
[Indexed for MEDLINE]

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