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Anal Bioanal Chem. 2019 Nov;411(29):7669-7680. doi: 10.1007/s00216-019-01959-z. Epub 2019 Jul 4.

Development and characterization of cellular biosensors for HTS of erythroid differentiation inducers targeting the transcriptional activity of γ-globin and β-globin gene promoters.

Author information

1
Department of Life Sciences and Biotechnology, Section of Biochemistry and Molecular Biology, University of Ferrara, Via Fossato di Mortara 74, 44121, Ferrara, Italy.
2
Biotechnology Center, University of Ferrara, 44121, Ferrara, Italy.
3
Department of Chemical and Pharmaceutical Sciences, University of Ferrara, 44121, Ferrara, Italy.
4
Department of Biomedical Sciences and Specialist Surgery, Section of Biochemistry, Molecular Biology and Medical Genetics, University of Ferrara, 44121, Ferrara, Italy.
5
Hematology Division, Children's Hospital of Philadelphia, Philadelphia, PA, 19104, USA.
6
IRBM Science Park SpA, 00071, Pomezia (Rome), Italy.
7
Department of Life Sciences and Biotechnology, Section of Biochemistry and Molecular Biology, University of Ferrara, Via Fossato di Mortara 74, 44121, Ferrara, Italy. gam@unife.it.

Abstract

There is a general agreement that pharmacologically mediated stimulation of human γ-globin gene expression and increase of production of fetal hemoglobin (HbF) is a potential therapeutic approach in the experimental therapy of β-thalassemia and sickle cell anemia. Here, we report the development and characterization of cellular biosensors carrying enhanced green fluorescence protein (EGFP) and red fluorescence protein (RFP) genes under the control of the human γ-globin and β-globin gene promoters, respectively; these dual-reporter cell lines are suitable to identify the induction ability of screened compounds on the transcription in erythroid cells of γ-globin and β-globin genes by FACS with efficiency and reproducibility. Our experimental system allows to identify (a) HbF inducers stimulating to different extent the activity of the γ-globin gene promoter and (b) molecules that stimulate also the activity of the β-globin gene promoter. A good correlation does exist between the results obtained by using the EGFP/RFP clones and experiments performed on erythroid precursor cells from β-thalassemic patients, confirming that this experimental system can be employed for high-throughput screening (HTS) analysis. Finally, we have demonstrated that this dual-reporter cell line can be used for HTS in 384-well plate, in order to identify novel HbF inducers for the therapy of β-thalassemia and sickle cell anemia. Graphical abstract.

KEYWORDS:

Erythroid differentiation; HTS; HbF inducers; Reporter systems; Sickle cell anemia; Thalassemia

PMID:
31273412
DOI:
10.1007/s00216-019-01959-z
[Indexed for MEDLINE]

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