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Development. 2019 Jul 22;146(14). pii: dev176735. doi: 10.1242/dev.176735.

Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation.

Author information

1
Department of Experimental and Health Sciences, Universitat Pompeu Fabra, 08003 Barcelona, Spain.
2
Goethe University, 60438 Frankfurt, Germany.
3
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
4
Department of Experimental and Health Sciences, Universitat Pompeu Fabra, 08003 Barcelona, Spain cristina.pujades@upf.edu.

Abstract

Cells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extracellular matrix, and to cell shape and actomyosin cytoskeletal changes. In this study, we demonstrate the role of Yap/Taz-TEAD activity as a sensor of mechanical signals in the regulation of the progenitor behavior of boundary cells during zebrafish hindbrain compartmentalization. Monitoring of in vivo Yap/Taz activity during hindbrain segmentation indicated that boundary cells responded to mechanical cues in a cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD boundary cells decreased their proliferative activity when Yap/Taz-TEAD activity ceased, which preceded changes in their cell fate from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining progenitor features in the hindbrain boundary cell population.

KEYWORDS:

Boundaries; Compartments; Hindbrain; Mechanical cues; Neurons; Progenitor cells; Yap/Taz

PMID:
31273051
DOI:
10.1242/dev.176735

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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