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Sci Total Environ. 2019 Nov 1;689:149-159. doi: 10.1016/j.scitotenv.2019.06.386. Epub 2019 Jun 25.

Concomitant exposure to benzo[a]pyrene and triclosan at environmentally relevant concentrations induces metabolic syndrome with multigenerational consequences in Silurana (Xenopus) tropicalis.

Author information

1
Univ. Grenoble-Alpes, Univ. Savoie Mont Blanc, CNRS, LECA, 38000 Grenoble, France.
2
Univ. Grenoble-Alpes, Univ. Savoie Mont Blanc, CNRS, LECA, 38000 Grenoble, France. Electronic address: sylvie.veyrenc@univ-grenoble-alpes.fr.
3
Univ. Grenoble-Alpes, INSERM, LBFA, 38000 Grenoble, France. Electronic address: karine.couturier@univ-grenoble-alpes.fr.
4
Univ. Grenoble-Alpes, INSERM, LBFA, 38000 Grenoble, France. Electronic address: cecile.batandier@univ-grenoble-alpes.fr.
5
Institut de Génomique Fonctionnelle de Lyon, Univ. Lyon 1, CNRS UMR5242, Ecole Normale Supérieure de Lyon, 69000 Lyon, France. Electronic address: anne-laure.bulteau@ens-lyon.fr.
6
Rovaltain Research Company, F26300 Alixan, France. Electronic address: dlejon@rovaltainresearch.com.
7
Rovaltain Research Company, F26300 Alixan, France. Electronic address: Bruno.combourieu@rovaltainresearch.com.
8
Centre de Ressources Biologiques Xénopes, Univ. Rennes 1, CNRS, UMS 3387 Rennes, France. Electronic address: thomas.lafond@univ-rennes1.fr.
9
Univ. Grenoble-Alpes, Univ. Savoie Mont Blanc, CNRS, LECA, 38000 Grenoble, France. Electronic address: muriel.raveton@univ-grenoble-alpes.fr.
10
Univ. Grenoble-Alpes, Univ. Savoie Mont Blanc, CNRS, LECA, 38000 Grenoble, France. Electronic address: stephane.reynaud@univ-grenoble-alpes.fr.

Abstract

Numerous studies suggest that amphibians are highly sensitive to endocrine disruptors (ED) but their precise role in population decline remains unknown. This study shows that frogs exposed to a mixture of ED throughout their life cycle, at environmentally relevant concentrations, developed an unexpected metabolic syndrome. Female Silurana (Xenopus) tropicalis exposed to a mixture of benzo[a]pyrene and triclosan (50 ng·L-1 each) from the tadpole stage developed liver steatosis and transcriptomic signature associated with glucose intolerance syndrome, and pancreatic insulin hyper secretion typical of pre-diabetes. These metabolic disorders were associated with delayed metamorphosis and developmental mortality in their progeny, both of which have been linked to reduced adult recruitment and reproductive success. Indeed, F1 females were smaller and lighter and presented reduced reproductive capacities, demonstrating a reduced fitness of ED-exposed Xenopus. Our results confirm that amphibians are highly sensitive to ED even at concentrations considered to be safe for other animals. This study demonstrates that ED might be considered as direct contributing factors to amphibian population decline, due to their disruption of energetic metabolism.

KEYWORDS:

Amphibian population decline; Endocrine disruptors; Metabolic syndrome; Silurana (Xenopus) tropicalis; Transgenerational

PMID:
31271984
DOI:
10.1016/j.scitotenv.2019.06.386
[Indexed for MEDLINE]

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