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Int J Mol Sci. 2019 Jul 2;20(13). pii: E3262. doi: 10.3390/ijms20133262.

Modulation of CAT-2B-Mediated l-Arginine Uptake and Nitric Oxide Biosynthesis in HCT116 Cell Line Through Biological Activity of 4'-Geranyloxyferulic Acid Extract from Quinoa Seeds.

Author information

1
Department of Psychological, Health and Territorial Sciences, University G. D'Annunzio, 66100 Chieti, Italy. sara.franceschelli@unich.it.
2
Department of Medicine and Science of Aging, University G. D'Annunzio, 66100 Chieti, Italy.
3
Department of Psychological, Health and Territorial Sciences, University G. D'Annunzio, 66100 Chieti, Italy.
4
Department of Physiology, Institute of Nutrition and Food Technology José Mataix, Biomedical Research Centre, University of Granada, 18071 Granada, Spain.
5
Department of Pharmacy, University Gabriele D'Annunzio of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti Scalo (CH), Italy.
6
Department of Medicine and Science of Aging, University G. D'Annunzio, 66100 Chieti, Italy. lorenza.speranza@unich.it.

Abstract

Chenopodium quinoa Wild is a "pseudocereal" grain which attracts a lot of attention in the scientific community as it has a positive effect on health. Here, we investigate the presence of biologically active O-prenylated phenylpropanoids in the ethanol extract of commercially available quinoa seeds. We claim that 4'-Geranyloxyferulic acid (GOFA) was the only phytochemical product found that belongs to quinoa's group secondary metabolites. We studied the changes in the oxidative and inflammatory status of the cellular environment in HCT 116 cell line processed with quinoa extract and its component GOFA; the implementation was done through the analysis of the antioxidant enzymes (SOD and CAT), the pro-inflammatory components (iNOS, IL-6 and TNF-α), and the products of intermediary metabolism (ONOO-, O2-). Moreover, the l-arginine uptake was proposed as a target of the tested compounds. We demonstrated that the GOFA, through a decrease of the CAT-2B expression, leads to a reduction of the l-arginine uptake, downregulating the harmful iNOS and restoring the altered redox state. These results propose a new molecular target involved in the reduction of the critical inflammatory process responsible for the cancer progression.

KEYWORDS:

CAT-2B; IL-6; TNF-α; inflammation; l-arginine; nitric oxide; oxidative stress

PMID:
31269760
DOI:
10.3390/ijms20133262
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