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Scand J Immunol. 2019 Jul 3:e12801. doi: 10.1111/sji.12801. [Epub ahead of print]

Functional Immune Response to Influenza H1N1 in Children and Adults after Live Attenuated Influenza Virus Vaccination.

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Influenza Centre, Department of Clinical Science, University of Bergen, Bergen, Norway.
K.G. Jebsen Centre for Influenza Vaccine Research, Department of Clinical Science, University of Bergen, Bergen, Norway.
Emergency Care Clinic, Haukeland University Hospital, Bergen, Norway.
Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Department of Research & Development, Haukeland University Hospital, Bergen, Norway.
Broegelmann Research Laboratory, Department of Clinical Science, University of Bergen, Norway.


Influenza virus is a major respiratory pathogen and vaccination is the main method of prophylaxis. In 2012, the trivalent live attenuated influenza vaccine (LAIV) was licensed in Europe for use in children. Vaccine-induced antibodies directed against the main viral surface glycoproteins, haemagglutinin (HA) and neuraminidase (NA), play important roles in limiting virus infection. The objective of this study was to dissect the influenza-specific antibody responses in children and adults, and T cells responses in children induced after LAIV immunization to the A/H1N1 viruses. Blood samples were collected pre- and at 28 and 56 days post-vaccination from 20 children and 20 adults. No increase in microneutralization (MN) antibodies against A/H1N1 were observed after vaccination. A/H1N1 stalk specific neutralizing and NA-inhibiting (NI) antibodies were boosted in children after LAIV. Interferon γ producing T cells increased significantly in children, and antibody-dependent cellular-mediated cytotoxic (ADCC) cell activity increased slightly in children after vaccination, although this change was not significant. The results indicate that the NI assay is more sensitive to qualitative changes in serum antibodies after LAIV. There was a considerable difference in the immune response in children and adults after vaccination, which may be related to priming and previous influenza history. Our findings warrant further studies for evaluating LAIV vaccination immunogenicity. This article is protected by copyright. All rights reserved.


LAIV ; Adults; Children; Immune response; Influenza A/H1N1


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