Send to

Choose Destination
Anticancer Agents Med Chem. 2019 Jul 2. doi: 10.2174/1871520619666190702120829. [Epub ahead of print]

Evaluation the Synergism Mechanism of Tamoxifen and Docetaxel by Nanoparticles.

Author information

College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, 310053. China.
College of Pharmacy, Liaoning University, Shenyang 110036. China.



Our previous studies have shown that the Docetaxel (DTX) and Tamoxifen (TMX) laoded nanoparticles(Co-NPs) could exibit synergistic effect on estrogen receptor positive cell lines. In the current study,we have studied synergistic effect of Co-NPs and underlying possible molecular mechanism.


Cell apoptosis assay,pharmacokinetic experiment and immunohistochemistry experiment were used to explore synergistic effect and underlying possible mechanism in vitro and in vivo.


Cell apoptosis assay revealed that Co-NPs could mediate cell sensitization to cytotoxic agent, resulting in a remarkable cell apoptosis. In addition, pharmacokinetic experiment research showed that Co-NPs have longer circulation time in vivo, which could prolong the treatment time of the chemotherapeutic drugs. Immunohistochemistry experiment revealed that the Co-NPs could downregulated the expression of P-gp level to reduce the drugs' efflux.


The possible mechanism of synergistic effect of DTX and TMX by Co-NPs was attributed to the longer in vivo circulation time, the significant increased rate of cell apoptosis and downregulated the expression of P-gp level to the tumor cells.


Cell apoptosis; Combination therapy ; Docetaxel ; P-glycoprotein ; Tamoxifen

Supplemental Content

Full text links

Icon for Bentham Science Publishers Ltd.
Loading ...
Support Center