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Methods Mol Biol. 2019;2013:83-90. doi: 10.1007/978-1-4939-9550-9_6.

Serological Profiling for Malaria Surveillance Using a Standard ELISA Protocol.

Author information

1
KEMRI Wellcome Trust Research Programme, Centre for Geographic Medicine Research-Coast, Kilifi, Kenya. LMurungi@kemri-wellcome.org.
2
KEMRI Wellcome Trust Research Programme, Centre for Geographic Medicine Research-Coast, Kilifi, Kenya.
3
Department of Biochemistry, Pwani University, Kilifi, Kenya.
4
Centre for Infectious Diseases, Heidelberg University Hospital, Heidelberg, Germany.

Abstract

The enzyme-linked immunosorbent assay (ELISA) is a reliable and relatively low-cost method for measuring soluble ligands such as antibodies and proteins in biological samples. For analysis of specific antibodies in serum, a capture antigen is immobilized onto a solid polystyrene surface from which it can capture the antibodies. The captured antibodies are subsequently detected using a secondary antibody conjugated to an enzyme. Detection is accomplished by addition of a colorimetric substrate, and the readout is absorbance (optical density). Here, we provide a detailed standardized ELISA protocol for the quantification of antibodies against malaria antigens.

KEYWORDS:

Antibodies; Antigens; ELISA; Optical density; Serum

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