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Nat Plants. 2019 Jul;5(7):731-741. doi: 10.1038/s41477-019-0444-6. Epub 2019 Jul 1.

A stigmatic gene confers interspecies incompatibility in the Brassicaceae.

Author information

1
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. a-fujii@mail.ecc.u-tokyo.ac.jp.
2
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan. a-fujii@mail.ecc.u-tokyo.ac.jp.
3
Japan Science and Technology Agency, Precursory Research for Embryonic Science and Technology, Saitama, Japan. a-fujii@mail.ecc.u-tokyo.ac.jp.
4
Department of Biology, Graduate School of Science, Chiba University, Chiba, Japan.
5
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
6
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan.
7
Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
8
Department of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland.
9
Kihara Institute for Biological Research, Yokohama City University, Yokohama, Japan.
10
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. a-taka@mail.ecc.u-tokyo.ac.jp.
11
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan. a-taka@mail.ecc.u-tokyo.ac.jp.

Abstract

Pre-zygotic interspecies incompatibility in angiosperms is a male-female relationship that inhibits the formation of hybrids between two species. Here, we report on the identification of STIGMATIC PRIVACY 1 (SPRI1), an interspecies barrier gene in Arabidopsis thaliana. We show that the rejection activity of this stigma-specific plasma membrane protein is effective against distantly related Brassicaceae pollen tubes and is independent of self-incompatibility. Point-mutation experiments and functional tests of synthesized hypothetical ancestral forms of SPRI1 suggest evolutionary decay of SPRI1-controlled interspecies incompatibility in self-compatible A. thaliana. Hetero-pollination experiments indicate that SPRI1 ensures intraspecific fertilization in the pistil when pollen from other species are present. Our study supports the idea that SPRI1 functions as a barrier mechanism that permits entrance of pollen with an intrinsic signal from self species.

PMID:
31263241
DOI:
10.1038/s41477-019-0444-6

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