High-yielding rice Takanari has superior photosynthetic response to a commercial rice Koshihikari under fluctuating light

Shunsuke Adachi; Yu Tanaka; Atsuko Miyagi; Makoto Kashima; Ayumi Tezuka; Yoshihiro Toya; Shunzo Kobayashi; Satoshi Ohkubo; Hiroshi Shimizu; Maki Kawai-Yamada; Rowan F Sage; Atsushi J Nagano; Wataru Yamori

doi:10.1093/jxb/erz304

High-yielding rice Takanari has superior photosynthetic response to a commercial rice Koshihikari under fluctuating light

J Exp Bot. 2019 Oct 15;70(19):5287-5297. doi: 10.1093/jxb/erz304.

Authors

Shunsuke Adachi^{1

2}, Yu Tanaka^{2

3}, Atsuko Miyagi⁴, Makoto Kashima⁵, Ayumi Tezuka⁵, Yoshihiro Toya⁶, Shunzo Kobayashi³, Satoshi Ohkubo¹, Hiroshi Shimizu⁶, Maki Kawai-Yamada⁴, Rowan F Sage⁷, Atsushi J Nagano^{2

8}, Wataru Yamori^{2

9}

Affiliations

¹ Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, Saiwaicho, Fuchu, Tokyo, Japan.
² Japan Science and Technology Agency, Precursory Research for Embryonic Science and Technology, Kawaguchi, Japan.
³ Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, Japan.
⁴ Graduate School of Science and Engineering, Saitama University, Shimo-Okubo, Sakura-ku, Saitama, Japan.
⁵ Research Institute for Food and Agriculture, Ryukoku University, Yokotani, Seta Oe-cho, Otsu, Shiga, Japan.
⁶ Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Yamadaoka, Suita, Osaka, Japan.
⁷ Department of Ecology and Evolutionary Biology, University of Toronto, Toronto, ON, Canada.
⁸ Faculty of Agriculture, Ryukoku University, Yokotani, Seta Oe-cho, Otsu, Shiga, Japan.
⁹ Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan.

Abstract

Leaves within crop canopies experience variable light over the course of a day, which greatly affects photosynthesis and crop productivity. Little is known about the mechanisms of the photosynthetic response to fluctuating light and their genetic control. Here, we examined gas exchange, metabolite levels, and chlorophyll fluorescence during the photosynthetic induction response in an Oryza sativa indica cultivar with high yield (Takanari) and a japonica cultivar with lower yield (Koshihikari). Takanari had a faster induction response to sudden increases in light intensity than Koshihikari, as demonstrated by faster increases in net CO2 assimilation rate, stomatal conductance, and electron transport rate. In a simulated light regime that mimicked a typical summer day, the faster induction response in Takanari increased daily CO2 assimilation by 10%. The faster response of Takanari was explained in part by its maintenance of a larger pool of Calvin-Benson cycle metabolites. Together, the rapid responses of electron transport rate, metabolic flux, and stomatal conductance in Takanari contributed to the greater daily carbon gain under fluctuating light typical of natural environments.

Keywords: Chlorophyll fluorescence; metabolome; photosynthesis; photosynthetic induction; rice; stomatal conductance; sunfleck; transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Light*
Oryza / growth & development
Oryza / metabolism*
Oryza / radiation effects
Photosynthesis*
Plant Leaves / metabolism*
Plant Leaves / radiation effects