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J Biosci Bioeng. 2019 Jun 25. pii: S1389-1723(19)30310-X. doi: 10.1016/j.jbiosc.2019.06.002. [Epub ahead of print]

Polyethyleneimine-induced astaxanthin accumulation in the green alga Haematococcus pluvialis by increased oxidative stress.

Author information

1
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan. Electronic address: t_yoshitomi@bio.c.u-tokyo.ac.jp.
2
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan; Graduate School of Chemical Sciences and Technology, Tokyo University of Science, 12-1 Ichigaya Funagawara-machi, Shinjuku-ku, Tokyo 162-0826, Japan.
3
Graduate School of Chemical Sciences and Technology, Tokyo University of Science, 12-1 Ichigaya Funagawara-machi, Shinjuku-ku, Tokyo 162-0826, Japan.
4
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan; JST PRESTO, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.

Abstract

The unicellular green microalga Haematococcus pluvialis accumulates large amounts of the red ketocarotenoid astaxanthin under stress conditions such as nitrogen deficiency. In this study, we discovered an astaxanthin accumulation in H. pluvialis cells by the addition of a synthetic cationic polymer, polyethyleneimine (PEI), into the cell culture. With an increase in PEI amount, amount of astaxanthin accumulation was increased. To investigate the mechanism for the accumulation of astaxanthin by the addition of PEI in H. pluvialis cells, we measured a localization of PEI in the cells and a production of reactive oxygen species. PEI was internalized in the cells through the negatively-charged cell walls, leading to excessive production of reactive oxygen species in the cells. Thus, the increased oxidative stress by cellular uptake of PEI resulted in the acceleration of astaxanthin accumulation in H. pluvialis.

KEYWORDS:

Astaxanthin; Cellular uptake; Flocculant; Haematococcus pluvialis; Microalgae; Oxidative stress; Polyethyleneimine; Reactive oxygen species

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