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Antonie Van Leeuwenhoek. 2019 Jun 27. doi: 10.1007/s10482-019-01293-5. [Epub ahead of print]

Streptococcus castoreus, an uncommon group A Streptococcus in beavers.

Author information

1
Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str. 17, 10315, Berlin, Germany. muehldorfer@izw-berlin.de.
2
Chemical and Veterinary Investigations Office Stuttgart, Schaflandstraße 3/2, 70736, Fellbach, Germany.
3
Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Giza Square, 12211, Egypt.
4
Hessian State Laboratory (LHL), Schubertstr. 60, 35392, Giessen, Germany.
5
Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Frankfurter Str. 85-89, 35392, Giessen, Germany.
6
Institute of Applied Microbiology, Justus Liebig University Giessen, Heinrich-Buff-Ring 26, 35392, Giessen, Germany.
7
German National Reference Center for Streptococci, Department of Medical Microbiology, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074, Aachen, Germany.
8
Landeslabor Berlin-Brandenburg, Gerhard-Neumann-Straße 2, 15236, Frankfurt (Oder), Germany.
9
Wilhelma - Zoological and Botanical Gardens, Wilhelma 13, 70342, Stuttgart, Germany.
10
Bavarian Health and Food Safety Authority, Veterinärstr. 2, 85764, Oberschleißheim, Germany.

Abstract

Streptococcus castoreus is a rarely encountered beta-haemolytic group A Streptococcus with high tropism for the beaver as host. Based on 27 field isolates under study, evidence strongly suggests that S. castoreus behaves as an opportunistic pathogen in beavers. Although it belongs to the resident mucosal microbiota, this Streptococcus species is associated with purulent lesions in diseased animals. With few exceptions, isolates proved to be highly similar in a panel of phenotypic (including biochemistry, resistance pattern, MALDI-TOF mass spectrometry and Fourier transform-infrared spectroscopy) and classic molecular (16S rRNA and sodA gene) analyses, and thus did not show any specific pattern according to host species or spatio-temporal origin. Conversely, S. castoreus isolates were differentiated into a multitude of pulsed-field gel electrophoresis 'pulsotypes' that did not seem to reflect true epidemiologic lineages. In contrast, single reactions of genomic fingerprinting using BOX-, (GTG)5- and RAPD-PCRs revealed at least subclusters with respect to host species, geographic origin or year, and confirmed the co-colonization of individuals with more than one isolate. In addition to isolates from free-ranging Eurasian beavers (Castor fiber), this study includes S. castoreus from captive North American beavers (Castor canadensis) for the first time.

KEYWORDS:

Bacteria; Beaver; Beta-haemolytic streptococci; Castor; Lancefield group A; MALDI-TOF MS; Pyogenic group

PMID:
31250158
DOI:
10.1007/s10482-019-01293-5

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