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Nat Commun. 2019 Jun 27;10(1):2828. doi: 10.1038/s41467-019-10773-1.

The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity.

Author information

1
Institute of Plant Biochemistry and Photosynthesis (IBVF-CSIC-University of Seville), Avenida Américo Vespucio 49, 41092, Seville, Spain.
2
Institute of Plant Biochemistry and Photosynthesis (IBVF-CSIC-University of Seville), Avenida Américo Vespucio 49, 41092, Seville, Spain. myriam.calonje@ibvf.csic.es.

Abstract

H2A.Z variant has emerged as a critical player in regulating plant responses to environment; however, the mechanism by which H2A.Z mediates this regulation remains unclear. In Arabidopsis, H2A.Z has been proposed to have opposite effects on transcription depending on its localization within the gene. These opposite roles have been assigned by correlating gene expression and H2A.Z enrichment analyses but without considering the impact of possible H2A.Z post-translational modifications. Here, we show that H2A.Z can be monoubiquitinated by the PRC1 components AtBMI1A/B/C. The incorporation of this modification is required for H2A.Z-mediated transcriptional repression through a mechanism that does not require PRC2 activity. Our data suggest that the dual role of H2A.Z in regulating gene expression depends on the modification that it carries, while the levels of H2A.Z within genes depend on the transcriptional activity.

PMID:
31249301
PMCID:
PMC6597585
DOI:
10.1038/s41467-019-10773-1
[Indexed for MEDLINE]
Free PMC Article

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