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Int J Ophthalmol. 2019 Jun 18;12(6):930-935. doi: 10.18240/ijo.2019.06.08. eCollection 2019.

Effects of astaxanthin on antioxidant parameters in ARPE-19 cells on oxidative stress model.

Author information

1
Department of Ophthalmology, Süleyman Demirel University Research and Education Hospital, Çünür-Isparta 32200, Turkey.
2
Department of Biophysics, Faculty of Medicine, Süleyman Demirel University Research and Education Hospital, Çünür-Isparta 32200, Turkey.

Abstract

AIM:

To observe the protective effect of astaxanthin (AST) against hydroquinone (HQ) mediated cell death in the apoptotic cascade and evaluate intracellular Ca2+ release, caspase-3, and -9 activation, reactive oxygen species (ROS) production in ARPE-19 cells.

METHODS:

We cultured ARPE-19 cells in special mediums and performed MTT tests to determine protective effect of AST, before exposing the cells to HQ in an incubator. We analyzed intracellular Ca2+ release experiments, mitochondrial membrane depolarization, glutathione (GSH), glutathione peroxidase (GSH-Px) and ROS experiments, and apoptosis assay.

RESULTS:

ROS production ranges depend on the amount of cell death. We computed the correlation between ROS ranges and cell death by 20,70-dichlorofluorescein fluorescence, and Ca2+ levels by Fura-2-AM. HQ-induced cell death found out to rise ranges of caspase-3 and -9, and mitochondrial depolarization. These three steps were delayed by AST management.

CONCLUSION:

ARPE-19 cells are avoided from HQ-induced ROS production and caspase-3 and -9 activation by AST. AST may limit the range of caspase synthesis, Ca2+ release and excess production of ROS with antiapoptotic effect. This study proposes a new therapeutic approach for the treatment of age-related macular degeneration.

KEYWORDS:

ARPE-19 cell; apoptosis; astaxanthin; oxidative stress

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