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Proc Natl Acad Sci U S A. 2019 Jul 9;116(28):14325-14330. doi: 10.1073/pnas.1906300116. Epub 2019 Jun 24.

PUCHI regulates very long chain fatty acid biosynthesis during lateral root and callus formation.

Author information

1
Unité Mixte de Recherche (UMR) Diversité Adaptation et Développement des Plantes, Institut de Recherche pour le Développement, Université de Montpellier, 34394 Montpellier Cedex 5, France.
2
Department of Pharmacological, Medical and Agronomical Biotechnology, University of Science and Technology of Hanoi, Cau Giay District, Hanoi, Vietnam.
3
University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom.
4
Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan.
5
Laboratoire de Biogénèse Membranaire, UMR 5200, CNRS, Université de Bordeaux, 33882 Villenave d'Ornon Cedex, France.
6
Institut Jean-Pierre Bourgin, UMR 1318, Institut National de la Recherche Agronomique, AgroParisTech, CNRS, Université Paris-Saclay, 78026 Versailles Cedex, France.
7
Department of Biology, Graduate School of Science, Kobe University, Kobe 657-8501, Japan.
8
Unité Mixte de Recherche (UMR) Diversité Adaptation et Développement des Plantes, Institut de Recherche pour le Développement, Université de Montpellier, 34394 Montpellier Cedex 5, France; laurent.laplaze@ird.fr soazig.guyomarch@ird.fr.

Abstract

Lateral root organogenesis plays an essential role in elaborating plant root system architecture. In Arabidopsis, the AP2 family transcription factor PUCHI controls cell proliferation in lateral root primordia. To identify potential targets of PUCHI, we analyzed a time course transcriptomic dataset of lateral root formation. We report that multiple genes coding for very long chain fatty acid (VLCFA) biosynthesis enzymes are induced during lateral root development in a PUCHI-dependent manner. Significantly, several mutants perturbed in VLCFA biosynthesis show similar lateral root developmental defects as puchi-1 Moreover, puchi-1 roots display the same disorganized callus formation phenotype as VLCFA biosynthesis-deficient mutants when grown on auxin-rich callus-inducing medium. Lipidomic profiling of puchi-1 roots revealed reduced VLCFA content compared with WT. We conclude that PUCHI-regulated VLCFA biosynthesis is part of a pathway controlling cell proliferation during lateral root and callus formation.

KEYWORDS:

VLCFA; callus formation; cell division; lateral root formation; morphogenesis

PMID:
31235573
PMCID:
PMC6628829
[Available on 2019-12-24]
DOI:
10.1073/pnas.1906300116

Conflict of interest statement

The authors declare no conflict of interest.

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