Format

Send to

Choose Destination
BMB Rep. 2019 Jun 25. pii: 4535. [Epub ahead of print]

Mitochondrial Genome Mutations in Mesenchymal Stem Cells Derived from Human Dental Induced Pluripotent Stem Cells.

Author information

1
Department of Convergence Medicine & Stem Cell Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, South Korea; Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, South Korea.
2
Department of Convergence Medicine & Stem Cell Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, South Korea.
3
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, South Korea.
4
Department of Dentistry, Gyeongsang National University School of Medicine, Institute of Health Science, Jinju 52828, South Korea.
5
Department of Pathology, Asan Institute for life sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Korea.

Abstract

Ethical and safety issues have rendered mesenchymal stem cells (MSCs) popular candidates in regenerative medicine, but their therapeutic capacity is lower than that of induced pluripotent stem cells (iPSCs). This study compared original, dental tissue-derived MSCs with re-differentiated MSCs from iPSCs (iPS-MSCs). CD marker expression in iPS-MSCs was similar to original MSCs. iPS-MSCs expressed higher in pluripotent genes, but lower levels in mesodermal genes than MSCs. In addition, iPS-MSCs did not form teratomas. All iPSCs carried mtDNA mutations; some shared with original MSCs and others not previously detected therein. Shared mutations were synonymous, while novel mutations were non-synonymous or located on RNA-encoding genes. iPS-MSCs also harbored mtDNA mutations transmitted from iPSCs. Selected iPS-MSCs displayed lower mitochondrial respiration than original MSCs. In conclusion, screening for mtDNA mutations in iPSC lines for iPS-MSCs can identify mutation-free cell lines for therapeutic applications.

PMID:
31234953
Free full text

Supplemental Content

Full text links

Icon for Korean Society for Biochemistry and Molecular Biology
Loading ...
Support Center