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Mol Cells. 2019 Jun 25. doi: 10.14348/molcells.2019.0116. [Epub ahead of print]

Nuclear UPF1 Is Associated with Chromatin for Transcription-Coupled RNA Surveillance.

Author information

1
Graduate Department of Bioconvergence Science and Technology, Dankook University, Yongin 16892, Korea.

Abstract

mRNA quality is controlled by multiple RNA surveillance machineries to reduce errors during gene expression processes in eukaryotic cells. Nonsense-mediated mRNA decay (NMD) is a well-characterized mechanism that degrades error-containing transcripts during translation. The ATP-dependent RNA helicase up-frameshift 1 (UPF1) is a key player in NMD that is mostly prevalent in the cytoplasm. However, recent studies on UPF1-RNA interaction suggest more comprehensive roles of UPF1 on diverse forms of target transcripts. Here we used subcellular fractionation and immunofluorescence to understand such complex functions of UPF1. We demonstrated that UPF1 can be localized to the nucleus and predominantly associated with the chromatin. Moreover, we showed that UPF1 associates more strongly with the chromatin when the transcription elongation and translation inhibitors were used. These findings suggest a novel role of UPF1 in transcription elongation-coupled RNA machinery in the chromatin, as well as in translation-coupled NMD in the cytoplasm. Thus, we propose that cytoplasmic UPF1-centric RNA surveillance mechanism could be extended further up to the chromatin-associated UPF1 and cotranscriptional RNA surveillance. Our findings could provide the mechanistic insights on extensive regulatory roles of UPF1 for many cellular RNAs.

KEYWORDS:

RNA surveillance; UPF1; nonsense-mediated mRNA decay; nuclear localization; transcription

PMID:
31234619
DOI:
10.14348/molcells.2019.0116
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