Format

Send to

Choose Destination
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2019 Jun 25;36(3):421-427. doi: 10.7507/1001-5515.201801019.

[Research of simulated microgravity regulate MC3T3-E1 cells differentiation through the nuclear factor-kappa B signaling pathway].

[Article in Chinese; Abstract available in Chinese from the publisher]

Author information

1
Institute of Medical Service and Technology, Academy of System Engineering, Academy of Military Sciences, Tianjin 300161, P.R.China.
2
Institute of Medical Service and Technology, Academy of System Engineering, Academy of Military Sciences, Tianjin 300161, P.R.China.z56787@sohu.com.

Abstract

in English, Chinese

In this study, we aim to investigat the effect of microgravity on osteoblast differentiation in osteoblast-like cells (MC3T3-E1). In addition, we explored the response mechanism of nuclear factor-kappa B (NF-κB) signaling pathway to "zero- g" in MC3T3-E1 cells under the simulated microgravity conditions. MC3T3-E1 were cultured in conventional (CON) and simulated microgravity (SMG), respectively. Then, the expression of the related osteoblastic genes and the specific molecules in NF-κB signaling pathway were measured. The results showed that the mRNA and protein levels of alkaline phosphatase (ALP), osteocalcin (OCN) and type Ⅰ collagen (CoL-Ⅰ) were dramatically decreased under the simulated microgravity. Meanwhile, the NF-κB inhibitor α (IκB-α) protein level was decreased and the expressions of phosphorylation of IκB-α (p-IκB-α), p65 and phosphorylation of p65 (p-p65) were significantly up-regulated in SMG group. In addition, the IL-6 content in SMG group was increased compared to CON. These results indicated that simulated microgravity could activate the NF-κB pathway to regulate MC3T3-E1 cells differentiation.

KEYWORDS:

bone morphogenetic proteins; nuclear factor-kappa B signaling pathway; osteoblast; osteoblast differentiation; simulated microgravity

Supplemental Content

Loading ...
Support Center