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Dev Cell. 2019 Aug 5;50(3):339-354.e4. doi: 10.1016/j.devcel.2019.05.033. Epub 2019 Jun 20.

Lysosomal Regulation of Inter-mitochondrial Contact Fate and Motility in Charcot-Marie-Tooth Type 2.

Author information

1
Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
2
Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA. Electronic address: dkrainc@nm.org.

Abstract

Properly regulated mitochondrial networks are essential for cellular function and implicated in multiple diseases. Mitochondria undergo fission and fusion events, but the dynamics and regulation of a third event of inter-mitochondrial contact formation remain unclear. Using super-resolution imaging, we demonstrate that inter-mitochondrial contacts frequently form and play a fundamental role in mitochondrial networks by restricting mitochondrial motility. Inter-mitochondrial contact untethering events are marked and regulated by mitochondria-lysosome contacts, which are modulated by RAB7 GTP hydrolysis. Moreover, inter-mitochondrial contact formation and untethering are further regulated by Mfn1/2 and Drp1 GTP hydrolysis, respectively. Surprisingly, endoplasmic reticulum tubules are also present at inter-mitochondrial contact untethering events, in addition to mitochondrial fission and fusion events. Importantly, we find that multiple Charcot-Marie-Tooth type 2 disease-linked mutations in Mfn2 (CMT2A), RAB7 (CMT2B), and TRPV4 (CMT2C) converge on prolonged inter-mitochondrial contacts and defective mitochondrial motility, highlighting a role for inter-mitochondrial contacts in mitochondrial network regulation and disease.

KEYWORDS:

Charcot-Marie-Tooth type 2; Mfn2; RAB7; TRPV4; endoplasmic reticulum; inter-mitochondrial contact; lysosome; mitochondria; mitochondria-lysosome contact; super-resolution imaging

PMID:
31231042
PMCID:
PMC6726396
[Available on 2020-08-05]
DOI:
10.1016/j.devcel.2019.05.033

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