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Arch Virol. 2019 Sep;164(9):2355-2358. doi: 10.1007/s00705-019-04328-4. Epub 2019 Jun 21.

A single amino acid change in hemagglutinin reduces the cross-reactivity of antiserum against an equine influenza vaccine strain.

Author information

1
Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan. nemoto_manabu@equinst.go.jp.
2
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
3
Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
4
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, WI, USA.

Abstract

Equine influenza virus is an important pathogen for the horse industry because of its economic impact, and vaccination is a key control measure. Our previous work suggested that a mutation at position 144 in the hemagglutinin of Florida sublineage clade 2 viruses reduces the cross-neutralizing activity of antiserum against a former vaccine strain. To confirm this suggestion, here, we generated viruses by reverse genetics. Antibody titers against the mutated viruses were one-tenth to one-sixteenth of those against the former vaccine strain. Our findings confirm that this single amino acid substitution reduces the cross-reactivity of antiserum against this former Japanese vaccine.

PMID:
31227892
DOI:
10.1007/s00705-019-04328-4
[Indexed for MEDLINE]

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