Format

Send to

Choose Destination
Nat Commun. 2019 Jun 21;10(1):2748. doi: 10.1038/s41467-019-10765-1.

Immature excitatory neurons develop during adolescence in the human amygdala.

Author information

1
Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, 94143, USA.
2
Department of Neurological Surgery, University of California, San Francisco, San Francisco, CA, 94143, USA.
3
Department of Neurology, University of California, San Francisco, San Francisco, CA, 94143, USA.
4
Laboratory of Comparative Neurobiology, Institute Cavanilles, University of Valencia, CIBERNED, 46980, Valencia, Spain.
5
Predepartamental Unit of Medicine, Faculty of Health Sciences, Universitat Jaume I, 12071, Castelló de la Plana, Spain.
6
Human Neuroanatomy Laboratory, School of Medicine and CRIB, University of Castilla-La Mancha, 02006, Albacete, Spain.
7
Department of Psychiatry, Rock Hall, University of California, San Francisco, San Francisco, CA, 94158-2324, USA.
8
Department of Pathology, University of California, San Francisco, San Francisco, CA, 94143, USA.
9
Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, 94143, USA. AlvarezBuyllaA@ucsf.edu.
10
Department of Neurological Surgery, University of California, San Francisco, San Francisco, CA, 94143, USA. AlvarezBuyllaA@ucsf.edu.

Abstract

The human amygdala grows during childhood, and its abnormal development is linked to mood disorders. The primate amygdala contains a large population of immature neurons in the paralaminar nuclei (PL), suggesting protracted development and possibly neurogenesis. Here we studied human PL development from embryonic stages to adulthood. The PL develops next to the caudal ganglionic eminence, which generates inhibitory interneurons, yet most PL neurons express excitatory markers. In children, most PL cells are immature (DCX+PSA-NCAM+), and during adolescence many transition into mature (TBR1+VGLUT2+) neurons. Immature PL neurons persist into old age, yet local progenitor proliferation sharply decreases in infants. Using single nuclei RNA sequencing, we identify the transcriptional profile of immature excitatory neurons in the human amygdala between 4-15 years. We conclude that the human PL contains excitatory neurons that remain immature for decades, a possible substrate for persistent plasticity at the interface of the hippocampus and amygdala.

PMID:
31227709
PMCID:
PMC6588589
DOI:
10.1038/s41467-019-10765-1
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center