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Int J Biol Sci. 2019 Apr 22;15(6):1113-1124. doi: 10.7150/ijbs.32358. eCollection 2019.

Hypoxia-Mimicking Cobalt-Doped Borosilicate Bioactive Glass Scaffolds with Enhanced Angiogenic and Osteogenic Capacity for Bone Regeneration.

Author information

1
Department of Orthopedics, South Campus of Shanghai Sixth People's Hospital Affiliated of Shanghai University of Medicine&Health Sciences, 279 zhouzhu road, Shanghai 220120, People's Republic of China.
2
Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
3
Laboratory for Advance Lubricating Materials, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, People's Republic of China.
4
Department of Orthopedic Surgery, Zhejiang Hospital, Hangzhou 310013, People's Republic of China.
5
School of Materials Science and Engineering, Tongji University, Shanghai 200092, People's Republic of China.
6
Shanghai Fengxian District Central Hospital Affiliated of Southern Medical University, Shanghai 201400, People's Republic of China.

Abstract

The osteogenic capacity of synthetic bone substitutes is will be highly stimulated by a well-established functional vascularized network. Cobalt (Co) ions are known that can generate a hypoxia-like response and stimulates the production of kinds of angiogenic factors. Herein, we investigated the mechanism of cobalt-doped bioactive borosilicate (36B2O3, 22CaO, 18SiO2, 8MgO, 8K2O, 6Na2O, 2P2O5; mol%) glass scaffolds for bone tissues repairing and blood vessel formation in the critical-sized cranial defect site of rats and their effects on the hBMSCs in vitro were researched. The scaffolds can control release Co2+ ions and convert into hydroxyapatite soaking in simulative body fluids (SBF). The fabircated scaffolds without cytotoxic strongly improves HIF-1α generation, VEGF protein secretion, ALP activity and upregulates the expression of osteoblast and angiogenic relative genes in hBMSCs. Eight weeks after implantation, the bioactive glass scaffolds with 3wt % CoO remarkablely enhance bone regeneration and blood vascularized network at the defective site. In conclusion, as a graft material for bone defects, low-oxygen simulated cobalt-doped bioactive glass scaffold is promising.

KEYWORDS:

bioactive glass scaffolds; blood vascularized network; bone regeneration; cobalt ions; hypoxia-like response

Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

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