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Macromol Biosci. 2019 Jun 21:e1900114. doi: 10.1002/mabi.201900114. [Epub ahead of print]

Preparation of Small-Diameter Tissue-Engineered Vascular Grafts Electrospun from Heparin End-Capped PCL and Evaluation in a Rabbit Carotid Artery Replacement Model.

Author information

1
School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, 100081, China.
2
Beijing Key Laboratory of Pre-clinic Research and Evaluation for Cardiovascular Implant Materials, Fuwai Hospital National Cardiovascular Center, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100037, China.
3
Department of Vascular Surgery, Xuanwu Hospital and Institute of Vascular Surgery, Capital Medical University, Beijing, 100053, China.

Abstract

Aiming to construct small diameter (ID <6 mm) off-the-shelf tissue-engineered vascular grafts, the end-group heparinizd poly(ε-caprolactone) (PCL) is synthesized by a three-step process and then electrospun into an inner layer of double-layer vascular scaffolds (DLVSs) showing a hierarchical double distribution of nano- and microfibers. Afterward, PCL without the end-group heparinization is electrospun into an outer layer. A steady release of grafted heparin and the existence of a glycocalyx structure give the grafts anticoagulation activity and the conjugation of heparin also improves hydrophilicity and accelerates degradation of the scaffolds. The DLVSs are evaluated in six rabbits via a carotid artery interpositional model for a period of three months. All the grafts are patent until explantation, and meanwhile smooth endothelialization and fine revascularization are observed in the grafts. The composition of the outer layer of scaffolds exhibits a significant effect on the aneurysm dilation after implantation. Only one aneurysm dilation is detected at two months and no calcification is formed in the follow-up term. How to prevent aneurysms remains a challenging topic.

KEYWORDS:

aneurysm; electrospinning; heparin; poly(ε-caprolactone); tissue-engineered vascular graft

PMID:
31222914
DOI:
10.1002/mabi.201900114

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