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Hua Xi Kou Qiang Yi Xue Za Zhi. 2019 Jun 1;37(3):253-259. doi: 10.7518/hxkq.2019.03.005.

[Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering].

[Article in Chinese; Abstract available in Chinese from the publisher]

Author information

1
Dept. of Orthodontics, Stoma-tological Hospital of Tianjin Medical University, Tianjin 300070, China.
2
Dept. of Spine Surgery, Tianjin Hospital, Tianjin 300211, China.
3
The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai Uni-versity, Tianjin 300071, China.

Abstract

in English, Chinese

OBJECTIVE:

This study aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels. This study also aimed to investigate the applicability of the hydrogels in cartilage tissue engi-neering.

METHODS:

Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2∶1, 1∶1 and 1∶2 were prepared. The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA com-posite hydrogels were evaluated. Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering.

RESULTS:

The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1∶1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility.

CONCLUSIONS:

The CMCS/OSA compound hydrogel prepared with a 1∶1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.

KEYWORDS:

carboxymethyl chitosan; cartilage tissue engineering; hydrogel; sodium alginate; umbilical cord mesenchymalstem cell

PMID:
31218857
DOI:
10.7518/hxkq.2019.03.005
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